NAD+ – 500mg

Buy NAD+ 500mg| 99% Purity| Third-Party Tested

NAD stands for Nicotinamide Adenine Dinucleotide, an essential coenzyme present in the cells of all living organisms. NAD+ plays a crucial role in energy metabolism and cellular signaling and supports redox reactions across metabolic pathways. Arthur Harden and William John Young discovered NAD+ in 1906 during research on yeast fermentation processes. NAD+ cycles between two forms, NAD+ and NADH, allowing efficient electron transfer inside cells. NAD+ serves as a substrate for essential enzymes, including sirtuins and PARPs. Sirtuins regulate gene expression and longevity pathways, while PARPs support DNA repair and cellular stress responses. In research settings, NAD+ is supplied as a high-purity lyophilized powder, typically reaching ≥99% purity verified by HPLC analysis. Researchers study NAD+ in aging, mitochondrial function, metabolism, and neuroprotection models. NAD+ is intended strictly for laboratory research and is not approved for human consumption or therapeutic use.

NAD+ Molecular Structure and Chemical Properties

NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme found in living cells. It is not a peptide. Instead, it consists of two nucleotides joined by a pyrophosphate bond. One nucleotide contains adenine, while the other contains nicotinamide. NAD+ plays an important role in cellular energy metabolism, redox reactions, and enzyme activity. It acts as an electron carrier by converting between its oxidized form (NAD+) and reduced form (NADH).

NAD+ Chemical Properties Table

These details agree with the PubChem chemical record.

NAD+ Mechanism of Action

In laboratory models, NAD+ functions as a key electron carrier, transferring hydride ions during redox reactions occurring in glycolysis, the TCA cycle, and oxidative phosphorylation. These processes support ATP production within mitochondria. NAD+ also maintains the NAD+/NADH ratio, which regulates cellular energy homeostasis and metabolic flux across biological pathways. Beyond redox activity, NAD+ acts as a consumable substrate for multiple enzyme systems.

Essential NAD+-Dependent Enzymes

Sirtuins (SIRT1–7)

Regulate gene expression, mitochondrial biogenesis, autophagy, and inflammatory signaling.

PARPs

Support DNA damage repair and chromatin remodeling processes.

CD38 and sARM1

Influence calcium signaling pathways and immune responses. Preclinical research indicates that NAD+ levels decline with aging and cellular stress, disrupting mitochondrial activity and repair systems. Experimental elevation of NAD+ levels restores metabolic balance and improves cellular resilience in laboratory models.  

NAD+ 100 mg and 750 mg

NAD+ is available in standardized research vial sizes to support flexible experimental design.

100 mg Vials

Suitable for small-scale in vitro assays, enzyme kinetics studies, and cell culture experiments, as well as early titration work in laboratory models.

750 mg Vials

Designed for larger or repeated experiments, including long-term in vivo research focused on metabolism and aging studies. Larger quantities allow cost-efficient experimental scaling. Both formats are supplied as a white lyophilized powder and require reconstitution using sterile water under controlled laboratory conditions.

NAD+ Research

NAD+ has been extensively investigated in preclinical research involving cellular systems and animal models, particularly in aging, metabolism, and disease mechanism studies. Natural NAD+ decline with age contributes to impaired mitochondrial function, reduced sirtuin activity, and increased inflammatory signaling. Experimental restoration of NAD+ levels improves metabolic signaling in research models. Studies indicate activation of autophagy and enhanced mitochondrial biogenesis following NAD+ elevation, along with improvements in oxidative metabolism and insulin sensitivity in experimental settings. Animal disease models suggest potential neuroprotective effects related to conditions such as Alzheimer’s and Parkinson’s disease, with some studies reporting extended healthspan outcomes. Research into NAD+ precursors such as NR and NMN continues in human trials, while direct NAD+ investigations largely remain preclinical. Ongoing studies examine NAD+ biosynthesis pathways, including salvage and Preiss-Handler pathways, as well as NAD+ depletion in cardiovascular and aging-related models.

How to Store and Handle NAD+

Store unopened NAD+ vials in a cool, dark environment. Long-term storage is recommended at −20°C, while sealed vials remain stable at 2–8°C. Protect the compound from moisture, light exposure, and repeated temperature fluctuations. After reconstitution, store solutions at 2–8°C and use within recommended timelines. Avoid repeated freeze–thaw cycles and always follow sterile laboratory handling procedures. Disclaimer:

NAD+ (Nicotinamide Adenine Dinucleotide) supplied by Orion Peptides is intended strictly for laboratory research and scientific investigation. It is not intended for human or animal consumption, self-administration, diagnostic procedures, or therapeutic applications.

Research-grade NAD+ sold by Orion Peptides is not an FDA-approved drug compound. Its safety, effectiveness, and suitability for clinical or personal use have not been established.

Referenced Citations

• Harden A, Young WJ (1906). Discovery of NAD+ as a fermentation cofactor.
• Lautrup S, et al. (2023). Roles of NAD+ in Health and Aging. Cell Metabolism. PMID: 37848251.
• Rajman L, et al. (2018). Therapeutic potential of NAD-boosting molecules. Cell Metabolism.
• Additional historical milestones and preclinical insights from physiology and metabolism research publications.